For cfp, 458nm is the best of our available laser wavelengths. Dichroic color tab 1 allows the user to switch between brightfield bf or fluorescence fluo 2. Leica sp8 tcs users manual follow the procedure for start up and log on as posted in the lab. If there is a gap, turn off the system lasers, mercury lamp, turn off the software but leave computer and hardware running. User guide to the ibif leica tcs sp8 mp confocal microscope. Manual x, y and z stage control is via the knobs attached to the. With this spectral information, any dye can be optimally excited with minimum crossexcitation and specimen. Aobs and wll 470nm670nm white light, pulsed laser source leica tcs sp8 x perfectly matches the wavelength of any fluorophore in the visible spectrum. The most important tools are located directly above the time line figure 9. Starting the lcs software on the leica tcs sp5 confocal microscope. To maintain information on the most current level, the description of software functions was intentionally omitted from this operating manual.
Introduction the ibif leica confocal microscope is made available on a feeforusehour basis to all users who have been trained. Leica tcs sp8 startup shutdown university of houston. The leica tcs sp5 is supplied with the latest version of the licensed las af software. Follow the procedure for start up and log on as posted in the lab. Further help is available via inprogram help within the leica lasaf software. This user manual specifies names of products or services that are trademarks or registered. The user can select the appropriate option for his or her application 1.
Leica sp8 confocal, sted, flim system microscopy and. This allows acquisition of excitation and emission spectra. Leica sp8 confocal with white light laser, falcon flim, sted and lightning deconvolution. Double click on the las x software found on the desktop. Sp8 lightning is a modular confocal microscope designed for optimal photon efficiency and high speed. By tuning both excitation and detection, complete excitation emission spectra can be acquired. Do not turn the lamp off if another user is scheduled within 2 hours after your use. Sted super resolution leica dm5000 cs leica dm6000 cs leica dm6000 cfs inverted leica dmi6000 cs leica dmi6000 cel. View online user manual manual for leica dmi8 microscope or simply click download button to examine the leica dmi8 guidelines offline on your desktop or laptop computer. Leica application and technology support group, life science division exton, pa 19341, usa leica tcs sp5 lasaf new user guide. Confocal application letter 3 fret with tcs sp8 fret wizards in leica application suite advanced fluorescence las af in the las af menu bar 1 you can find two wizards for performing fret experiments. Please log on with your account only and do not share your. Usermanual for leica sp8 confocal universitetet i bergen.
A modular design concept and an inclusive upgrade path ensure that each system, regardless of its configuration, can benefit from fundamental product features such as the fastest true confocal scanner all the way up to leica super. Training guide for leica sp8 confocalmultiphoton microscope. Description leica tcs sp8 has been designed for confocal microscopy with optimal photon efficiency and high speed. Select the fretab wizard from the tcs sp8 pulldown menu. Nih image free image processing and analysis program for. Leica tcs sp8 sted 3x confocal molecular imaging center. Leica dmi6000 inverted microscope instruction manual. Manual stage or custom made setup for animal fixation. All rights to this document are held by leica microsystems cms gmbh. Leica tcs sp8 wll confocal smd microscope wellcome.
Microscopes microscope stand options upright leica dm6 cs leica dm6 cfs leica dm6 cfs wo tl axis inverted leica dmi8 cs leica dmi8 cel compact leica dmi8 cel advanced vibration isolation isolation options speci. Leica dmi6000 inverted microscope brochure leica dmi6000 inverted microscope instruction manual. In addition, the leica tcs sp8 features 3 hyds hybrid detectors combining the characteristics of a classic pmt with a highly sensitive avalanche photodiode and 1 pmt which can measure real time response of timecritical sequences due to its fast scan speed. Leica tcs sp8 lives up to the high demands of this microscope discipline and can adapt as research evolves. The leica tcs sp8 sted 3x leica microsystems, germany is a highly versatile and advanced high end system from leica microsystems equipped with three hyds, two pmts and one transmission detector, tandem max speed 1800 hz and resonance 8000 hz scanners, lasers throughout the visible range, 2 depletion lasers for sted, and a sted 3d module. Leica tcs sp8 option machineposter en pdf, 728 kb sp8 scan head with 4tuneposter rgb may2018 pdf, 717 kb press releases. Introduction the ibif confocal microscope is made available on a feeforusehour basis to all users who have been trained. If you do not require high speed scanning leave resonant set to off. Leica sp8 user manuals and brochures download the leica tcs sp8 quick start poster and cores abridged instructions for getting started here. The gain setting as discussed elsewhere in this manual controls.
This will create individual directories that can be viewed only by the respective user. Unlike our previous instrument, it has a number of hardware features, specifically the objectives, the stage, and the condensers, that can be added or removed by the user, with the. Leica sp8 confocal with white light laser, falcon flim. All optical components are matched towards preserving fluorescence photons for image contrast and to improve cell viability in live cell imaging. The owneroperator and user of this product are responsible for proper. The leica tcs is a universal laser scanning confocal microscope system for the. User guide to the ibif leica tcs sp8 mp confocal microscope this version.
Settings here allow the user to control how the image is viewed on screen. Leica tcs sp8 x with white light laser wll perfectly matches the wavelength of any fluorophore. Then turn on the power supply for the scanner 1 and the lasers 2 by. If you turn it on you will not be able to use slower speeds. If you are the last user fo the day, turn off the complete system incl. In this example i have used a linked cfpyfp fret sensor with fret as its default state, expressed in the mushroom body of an adult drosophila brain. Page 1 user guide to the ibif leica tcs sp8 mp confocal microscope this version. View and download leica tcs sp8 mp user manual online. Quick start leica tcs sp8 system start up image acquisition zseries acquisition system shut down software start up laser start up turn on the microscope control box. This laser based system is ideal for fixed photostable applications where high confocal resolution is critical.
User manual leica tcs sp8 leica tcs sp8 mp leica tcs sp8 x. The existing multiuser confocal system, a leica tcs sp2 purchased in 2002 has been in continuous operation for over a decade and provided outstanding service to the research community. The leica tcs sp8 dls is a digital light sheet microscope, which is fully integrated into the leica tcs sp8 confocal microscope for the observation of. Tcs sp8 smd system variants with white light laser. Turn the pc microscope, scanner power, laser power, and the laser emission key to on bottom right of desk. Leica tcs sp8 confocal microscope system andrew lackner. It achieves this level of performance using highspeed resonant scanning with imaging formats of up to 2496 x 2496 pixels. The first step is to set the lasersdetectors for the fret donor. Leica tcs sp8 with compact supply unit and upright stand 1 research microscope 2. Page 1 training guide for leica sp8 confocalmultiphoton microscope las af v3. Up to eight excitation lines can be used simultaneously. Leica sp8 tcs users manual v2 university of oklahoma. Leica sp8 mp scientific center for optical and electron.
Leica lasx is the standard software used for the leica microscopes. If you have any suggestions or improvements for this user manual, please contact the leica branch office in your country. Fret ab and fret ratio leica sp8 university of houston. Turn on the fluorescent lamp top left of the computer monitor. It is recommended setting up a separate user id for each user system administrator.
Las x live data mode recording of manual and automated workflows, trigger functions, complex timelapse series. Unlike our previous instrument, it has a number of hardware features, specifically the. While the leica tcs sp2 was stateoftheart when purchased, the system no longer meets our needs with respect to multilabel imaging studies, photosensitivity. The sp8 lightning allows you to perform proper sampling over a specimen area at least six times larger than with any other confocal system. A pulsed 440nm laser and a continuous wave 405nm laser line is included for imaging of uvexcitable dyes. Quick start leica tcs sp8 florida state university. Leica dm2500 upright fluorescent microscope manuals.
Leica tcs sp8 mp look deeper into tissues with multiphoton microscopy 30 leica tcs sp8 cars imaging native specimens without dyes 32 leica hcs a amplify the power of screening 34 leica tcs sp8 smd learn more from single molecules 36 find the right leica tcs sp8 for your research 38 captures and acknowledgements 39. Leica dmi8 microscope user manual manual pdf viewdownload. Theres also the full leica sp8 user manual plus the other leica sp8 confocal manuals. How to do a tile scan with the leica dvm6 digital microscope duration. Leica histocore arcadia h laboratory equipment user manual. Leicatcs the confocal microscope system for highest demands in multi user facilities. Leica tcs sp8 technical documentation life science research division.
Remove the dust cover from the microscope turn on the mercury lamp a if you need to see the fluorescence through the eye pieces. Check the scheduling calendar to see if anyone else has signed up to use the leica sp8 after. This application letter describes work with the fret ab wizard 2. Leica tcs sp8 confocal microscope flow cytometrycell. The leica tcs sp2 system was designed for confocal recording and quantitative measurement via laser point scanning of fluorescence labeled living and fixed specimens. Since the lcs software is based on the user administration of windows. A guide through the live data mode live data mode with leica tcs sp8 7 4 tools in detail 4. Las af live data mode recording of manual and automated work.